When you say disabled, are you referring to the Hotspot prefab itself (determined by it's layer), or the Interactions within it? What is your Interaction method?
Icons will show so long as the Hotspot itself is highlight-able. This will happen even if no Interactions are enabled. To de-activate a Hotspot completely, use the Object: Send message Action to send "Turn off" to it. "Turn on" will re-activate it again.
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